Page 139 - Handbook on Food Fortification in Kenya
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Select randomly two daily-composite       a) Qualitative tests: Show simply the
samples and send to an external              presence or absence of an added
reference laboratory for the quantitative    micronutrient. An example is the black
determination of iron and vitamin A. The     light (uv light) test for riboflavin. This
frequency of this analysis will depend on    type of test is used to see if a flour
factory tonnage as follows:                  sample has been fortified or not.

(i) If production is less than 20 MT per 	   b) Semi quantitative tests: Gives a
      day, take 2 samples every 6 months.    rough indication of the level of an added
                                             nutrient. Examples are the iron spot test
(ii) For production 20-50 MT, test 2 	       and a color test recently developed for
      samples every 3 months and             vitamin A in flour. This type of test tells
                                             whether the level added is low, normal or
(iii) For production above 50 MT, test 2     high. 	 For example the iron spot test
      composite samples every month
                                             c) Quantitative tests: Gives an actual
Sampling plan- Oils                          value for the level of micronutrient
Fortificant: At least once a month,          in the sample. Quantitative tests
take two 30g samples from the                generally measure total content or
cans and send them to an external            both the natural and added levels.
laboratory to confirm the vitamin A.
                                             Iron spot test
Product: Prepare a composite sample          The iron spot test is universally
by collecting 200 mL of oil every hour,      used by Millers to check whether
and placing in an opaque 2-Ltr container.    flour has been properly fortified.

Semi-quantitative analysis should
be done hourly for each sample.

In the laboratory, mix well and take

50g to use for the determination

of retinol concentration using the                                                Food
                                                                                  sample
“Semi-quantitative  method  for                                                   results
determining retinol in fortified oil”.(8) 8

Analytical testing                                           Fe (ppm)
Samples should be tested to verify           Figure 34. - Iron spot test results
that it has been properly fortified.
Three types of testing are possible:

8 – Guamuch, Monica, Phillip Makhumula, and Omar Dary. “Food Control Manuals - Laboratory
Methods For Fortified Foods - Part II”. Ecsahc.org. N.p., 2016. Web. 13 Apr. 2016.

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